General Information

  • ID:  hor005333
  • Uniprot ID:  Q7LZ09
  • Protein name:  Lebetin-2-alpha
  • Gene name:  NA
  • Organism:  Macrovipera lebetina (Levantine viper) (Vipera lebetina)
  • Family:  natriuretic peptide family
  • Source:  animal
  • Expression:  Expressed by the venom gland.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Macrovipera (genus), Viperinae (subfamily), Viperidae (family), Colubroidea (superfamily), Serpentes (infraorder), Toxicofera, Episquamata, Unidentata, Bifurcata, Squamata (order), Lepidosauria (class), Sauria, Sauropsida, Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0090729 toxin activity
  • GO BP:  GO:0007165 signal transduction; GO:0008217 regulation of blood pressure; GO:0035821 modulation of process of another organism
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  GDNKPPKKGPPNGCFGHKIDRIGSHSGLGCNKVDDNKG
  • Length:  38
  • Propeptide:  GDNKPPKKGPPNGCFGHKIDRIGSHSGLGCNKVDDNKG
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  3-3N->A: Little decrease of antiplatelet activity.; 4-4N->A: Little decrease of antiplatelet activity.; 5-5N->A: Little decrease of antiplatelet activity.; 6-6N->A: Little decrease of antiplatelet activity.; 7-7N->A: Little decrease of antiplatelet activi

Activity

  • Function:  [Lebetin-1-alpha]: Inhibits platelet aggregation induced by thrombin, collagen and PAF-acether (PubMed:8769304, PubMed:9839678). Human platelet aggregation induced by thrombin is inhibited by synthetic lebetin-1-alpha with (IC(50)=140 nM) (PubMed:11910186). In vivo, inhibits collagen-induced thrombocytopenia in rats (PubMed:8769304, PubMed:9839678). Is not toxic upon intravenous injection into mice and rats (PubMed:8769304).
  • Mechanism:  Mutagenesis was carried out on lebetin-1-gamma.
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: induced human platelet aggregation ：2.5 nM
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  14-30
  • Structure ID:  AF-Q7LZ09-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q7LZ09-F1.pdbhor005333_AF2.pdbhor005333_ESM.pdb

Physical Information

Mass: 460556 Formula: C166H267N55O53S2
Absent amino acids: AEMQTWY Common amino acids: G
pI: 9.51 Basic residues: 9
Polar residues: 16 Hydrophobic residues: 5
Hydrophobicity: -128.16 Boman Index: -9392
Half-Life: 30 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 38.42
Instability Index: 2398.42 Extinction Coefficient cystines: 125
Absorbance 280nm: 3.38

Literature

  • PubMed ID:  8769304
  • Title:  Novel anti-platelet aggregation polypeptides from Vipera lebetina venom: isolation and characterization.
  • PubMed ID:  9839678
  • Title:  Anti-platelet activity of the peptides composing the lebetin 1 family, a new class of inhibitors of platelet aggregation.
  • PubMed ID:  11910186
  • Title:  Lebetin peptides: potent platelet aggregation inhibitors.